Protection of negative gravitaxis in Euglena gracilis Z against gamma-ray irradiation by Trolox C.

نویسندگان

  • Tetsuya Sakashita
  • Masahiro Doi
  • Hiroshi Yasuda
  • Shoichi Fuma
  • Donat-P Häder
چکیده

The protective effects of Trolox on the inhibition of negative gravitaxis in Euglena gracilis exposed to 200 Gy 60Co gamma-rays were examined using different concentrations (1, 10 and 100 microM). The orientation precision of the negative gravitaxis was quantified using the r-value. A significant decrease in the r-value was observed in gamma-irradiated samples (0.18+/-0.03) compared to those of non-irradiated samples (0.47+/-0.03). There were no significant changes in the r-value of cells exposed to 200 Gy gamma-rays by the addition of 1 or 10 microM of Trolox. A significant increase (0.19) in the r-value of cells exposed to 200 Gy with 100 microM Trolox was observed. The results indicates that Trolox at a concentration of 100 microM protects negative gravitaxis against 60Co gamma-ray irradiation at a dose of 200 Gy. It also suggests that the negative gravitaxis of Euglena gracilis is affected by free radicals.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

High Light-induced Sign Change of Gravitaxis in the Flagellate Euglena gracilis is Mediated by Reactive Oxygen Species

Euglena gracilis responds to abiotic stress factors (high light, salinity, heavy metals) with a sign change of its gravitactic behavior. This phenomenon is oxygen dependent and can be suppressed by the application of the reductant dithionite. It is not mediated by the photoreceptor since also blind mutants change their movement behavior upon high light exposure. It is also not mediated by the c...

متن کامل

Gamma-irradiation effect: variation of photosynthetic activity of Euglena.

OBJECTIVE To study the effects of gamma-ray irradiation on carbon fixation (Specific production rate: SPR), CO2 utilization efficiency (CUE) and electron transfer rate (ETR) in the photosynthetic flagellate Euglena gracilis strain Z in a dose-response dependent manner. METHODS Euglena cells were cultured in an inorganic nutrient medium containing ammonium chloride or proteose peptone. Cells w...

متن کامل

Protective effects of famotidine and vitamin C against radiation induced cellular damage in mouse spermatogenesis process

Background: Radioprotective effect of famotidine was previously shown on radiation induced micronuclei and chromosomal aberrations in human peripheral lymphocytes and mouse bone marrow cells however, its radioprotective property has never been studied in mouse spermatogenesis. It was also shown that vitamin C as an antioxidant also exert its radioprotective effect on many biological s...

متن کامل

The Carotenoid Hydrocarbons of Euglena gracilis and Derived Mutants.

An examination of the carotene fractions extracted from Euglena gracilis Z and pressure-bleached Euglena mutants PR-1, PR-2, PR-3, and PR-4 revealed phytoene in mutants PR-1, PR-2, and PR-3. Photosynthetic E. gracilis Z cultured at different light intensities showed no detectable phytoene, nor was phytoene found in mutant PR-4. However, dark-cultured E. gracilis Z yielded readily assayable amou...

متن کامل

Ultraviolet Sensitive Factors in the Cytoplasm That Affect the Differentiation of Euglena Plastids

The bleaching phenomenon in Euglena gracilis strain z offers a unique possibility for investigating the hereditary nature of plastids. Trea tment of Euglena cultures with streptomycin, elevated temperature, or irradiation with a mild close of U V light causes the permanent loss of the capacity to develop chloroplasts in the growing cultures. Early explanations of this phenomenon were that the b...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of radiation research

دوره 43 Suppl  شماره 

صفحات  -

تاریخ انتشار 2002